Automated Systems: Dynex DSX - ELISA automation
The DSXâ„¢ (code DY65400) Automated ELISA System is a computer-controlled microplate processing system that fully automates ELISA assays. The DSX System automates the sample distribution, incubation, reagent addition, washing, and detection phases of microplate assays.
It is intended for use in clinical, research, and industrial laboratories.
The DSX is an automated system that is useful for medium throughput, multiple assay applications. The system is designed to process specimens in a continuous flow from sample to result.
A run can contain up to 96 samples. Serum and/or plasma specimens are generally used for clinical testing, although other fluids such as urine or spinal fluid can be run. Specimens containing large particulate matter, such as stool, tissue homogenates, or culture media, can also be used. It is recommended that these specimens be processed through a mesh filter prior to loading onto the system to assure sample homogeneity and pipetting precision.
Sample IDs can be manually entered by the operator or they can be read automatically from barcode labels on the sample tubes.
A wide variety of standards or control samples can also be run. Standards or controls are loaded onto a separate rack that contains up to 33 standard/ control tubes. The tubes are available from your supplier.
The DSX System utilises a single reagent rack that contains up to 24 25ml reagent bottles. The required reagent bottles are available from your supplier.
Pipetting of samples, standards/controls, and reagents is performed using custom-designed disposable pipette tips to assure pipetting precision and eliminate possible cross-contamination.
Reagent tips pipette from 25 Î¼l to 1 ml of reagents, and sample tips pipette from 5 Î¼l to 300 Î¼l of samples or standards/controls.
The DSX System will perform single-stage or multi-stage sample dilutions. Dilutions ranging from 1:2 to 1:36,100 can be performed, with deep-well dilution plates or wells used for dilutions above 1:60.
The DSX can accommodate up to four temperature-controlled plate incubators. Each incubator can be set to a different temperature ranging from ambient plus 7Â°C to 50Â°C, and each plate can be shaken during incubation. The particular incubator modules that are used during a worklist, the temperature of each incubator, and the shake parameters are automatically set by the system when the worklist is created based on the assays in use.
Eight wells in one column of a 96-well plate can be washed simultaneously. Washing protocols can be defined so that all of the columns are washed in the same manner, or different wash cycles can be applied to specified plates.
A wide variety of user-defined wash protocols can be programmed on the system. In addition, different plate types can be accommodated.
During operation, each microplate is automatically transferred to the absorbance module at the appropriate time. The optical densities of the wells specified during assay definition are read, the various calculations (for example: blanking, QC of raw data, threshold or curve fitting) are applied, and the calculated results for the microplate are reported.
The DSXâ„¢ Automated ELISA System has a number of performance and convenience features.
These are summarized below:
Different assays can be run on the same plate
ESPâ„¢ - Electronic Signature Pipetting is available for detection of gross pipetting errors
Automatic reading of Sample IDs and microplate IDs from barcode labels
Endpoint data analysis to perform qualitative and quantitative data reduction
Less than 10 second reading time (using single wavelength)
On-board self diagnostics
Selection of up to six filters (405-450-490-630)
Single and dual wavelength reading modes
Easily removable incubator, wash, and absorbance modules for servicing
A variety of wash protocols can be programmed
A variety of plate types can be programmed
Liquid level sensing on liquid waste container and wash buffer containers
Aspirating/pipetting speed can be changed for viscous liquids
Dimensions and Weight
Depth < 91 cm (35.8 in)
Width < 106 cm (41.8 in)
Height < 80 cm (31.5 in)
Weight < 110 Kg (243 lb)
Samples: 96 samples (98 positions are available)
Controls and/or Standards: 33
Sample Pipetting Tips: 432
Reagent Pipetting Tips: 41
Wash Buffer Bottles: 4 bottles, 2 liters each
Waste Container: 8 liters
Ambient Drawer Module Incubation Temperatures Ambient plus 5Â°C
Incubator Module Incubation Temperatures Ambient plus 7Â°C to 50Â°C
Individual, paired or average wells
Whole plate or last plate
Row or column
Each well on the plate
Wavelength Modes: single, dual or multiple
Standard Curves: linear, quadratic, cubic, quartic, spline, polygon, sigmoid or Akima
Additional Data Analysis: threshold, ratio, QC equations
Flexible Template: up to eight different well types
Main Unit: 100 - 240 V (voltage), 800 VA (power), 50/60 Hz (frequency)
Line Voltage Variation: Â± 10%
Line Frequency Variation: Â± 3 Hz
Operating Range: 15Â°C to 30Â°C
15% to 85% relative humidity (non-condensing)
2000 Meters Altitude
Ports: RS232 serial port
Baud Rate: 19200. Character format.
Character Format: 7 data bits, 1 stop bit, no parity
The instrument is designed in accordance with IEC/EN 61010-1:2001 (2nd Edition), IEC 61326-1:2006, FCC 15: Part B and ICES-003 for Class A device.
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